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GAU8 wrote: ↑29/11/2020 23:46 Da si makar ravnozemljaš..
Ali jok moraš ti u antivaksere...

GAU8 wrote: ↑29/11/2020 23:46 Da si makar ravnozemljaš..
Ali jok moraš ti u antivaksere...

drag_gost wrote: ↑30/11/2020 08:49 Obrazovanje kolega, obrazovanje, nesto sto ti evidentno nedostaje u toj matrici.

drag_gost wrote: ↑30/11/2020 08:55 Sta bolan tvrdis, da je ljudski intervejs preko cega se daju nanorobotima komande nesto sto se desava? Pozdravljam rad na moralu, etici, sirokoumnosti, ali bez konvencionalnog obrazovanja to malo teze ide.

Danska premijerka Mette Frederiksen rasplakala se u četvrtak kada je posjetila uzgajivača nerčeva koji je izgubio svoje životinje nakon vladine odluke da se pobije 17 miliona nerčeva kako bi se spriječilo širenje korona virusa.

Lao Ce wrote: ↑30/11/2020 08:45

GAU8 wrote: ↑29/11/2020 23:46 Da si makar ravnozemljaš..
Ali jok moraš ti u antivaksere...

Čitaj malo za promjenu, nemoj samo lijepiti ljudima etikete bez ikakvog povoda i glumiti neku forumsku budalu po svaku cijenu. Konstantan rad na samome sebi, na emocijama, na mentalnom planu, na moralu i lijepom ponašanju, na zdravoj komunikaciji sa drugim ljudima itd, i ako Bog da nadat se da će jednog dana iz tebe ili nekih od vas izrasti neki insan. Upristoji se , nemoj biti hajvan.

SUMMARY CATALOGUE OF ERRORS FOUND IN THE PAPER
The Corman-Drosten paper contains the following specific errors:

1. There exists no specified reason to use these extremely high concentrations of primers in this protocol. The described concentrations lead to increased nonspecific bindings and PCR product amplifications, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

2. Six unspecified wobbly positions will introduce an enormous variability in the real world laboratory implementations of this test; the confusing nonspecific description in the Corman-Drosten paper is not suitable as a Standard Operational Protocol making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

3. The test cannot discriminate between the whole virus and viral fragments. Therefore, the test cannot be used as a diagnostic for intact (infectious) viruses, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus and make inferences about the presence of an infection.

4. A difference of 10° C with respect to the annealing temperature Tm for primer pair1 (RdRp_SARSr_F and RdRp_SARSr_R) also makes the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

5. A severe error is the omission of a Ct value at which a sample is considered positive and negative. This Ct value is also not found in follow-up submissions making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

6. The PCR products have not been validated at the molecular level. This fact makes the protocol useless as a specific diagnostic tool to identify the SARS-CoV-2 virus.

7. The PCR test contains neither a unique positive control to evaluate its specificity for SARS-CoV-2 nor a negative control to exclude the presence of other coronaviruses, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

8. The test design in the Corman-Drosten paper is so vague and flawed that one can go in dozens of different directions; nothing is standardized and there is no SOP. This highly questions the scientific validity of the test and makes it unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

9. Most likely, the Corman-Drosten paper was not peer-reviewed making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

10. We find severe conflicts of interest for at least four authors, in addition to the fact that two of the authors of the Corman-Drosten paper (Christian Drosten and Chantal Reusken) are members of the editorial board of Eurosurveillance. A conflict of interest was added on July 29 2020 (Olfert Landt is CEO of TIB-Molbiol; Marco Kaiser is senior researcher at GenExpress and serves as scientific advisor for TIB-Molbiol), that was not declared in the original version (and still is missing in the PubMed version); TIB-Molbiol is the company which was “the first” to produce PCR kits (Light Mix) based on the protocol published in the Corman-Drosten manuscript, and according to their own words, they distributed these PCR-test kits before the publication was even submitted [20]; further, Victor Corman & Christian Drosten failed to mention their second affiliation: the commercial test laboratory “Labor Berlin”. Both are responsible for the virus diagnostics there [21] and the company operates in the realm of real time PCR-testing.

In light of our re-examination of the test protocol to identify SARS-CoV-2 described in the Corman-Drosten paper we have identified concerning errors and inherent fallacies which render the SARS-CoV-2 PCR test useless.

CONCLUSION
The decision as to which test protocols are published and made widely available lies squarely in the hands of Eurosurveillance. A decision to recognise the errors apparent in the Corman-Drosten paper has the benefit to greatly minimise human cost and suffering going forward.

Is it not in the best interest of Eurosurveillance to retract this paper? Our conclusion is clear. In the face of all the tremendous PCR-protocol design flaws and errors described here, we conclude: There is not much of a choice left in the framework of scientific integrity and responsibility.

GAU8 wrote: ↑30/11/2020 09:11

Lao Ce wrote: ↑30/11/2020 08:45

GAU8 wrote: ↑29/11/2020 23:46 Da si makar ravnozemljaš..
Ali jok moraš ti u antivaksere...

Čitaj malo za promjenu, nemoj samo lijepiti ljudima etikete bez ikakvog povoda i glumiti neku forumsku budalu po svaku cijenu. Konstantan rad na samome sebi, na emocijama, na mentalnom planu, na moralu i lijepom ponašanju, na zdravoj komunikaciji sa drugim ljudima itd, i ako Bog da nadat se da će jednog dana iz tebe ili nekih od vas izrasti neki insan. Upristoji se , nemoj biti hajvan.

Komunikacija s drugim ljudima uvijek.
Ali sa ravnozemljasem, ili jos gore, antivakserom, to ne postoji

spinel_ wrote: ↑30/11/2020 09:01 https://6yka.com/novosti/danska-premije ... ni-nercevi

Danska premijerka Mette Frederiksen rasplakala se u četvrtak kada je posjetila uzgajivača nerčeva koji je izgubio svoje životinje nakon vladine odluke da se pobije 17 miliona nerčeva kako bi se spriječilo širenje korona virusa.

Ovo je grozno sta se radi a sve zbog navodne brige za drustvom.

kritikos wrote: ↑30/11/2020 09:46 Ono sto bih ja uveo zakonom u medijski prostor ja RATING novinara. Svaki tekst bi morao biti objavljen pod punim imenom i prezimenom, fotografijom i ratingom relevantnosti/tacnosti informacija koje je do sada prenio/objavio.
Ovo danas je zlocin od novinara sta rade na mrezama/medijima a ne snose nikakvu odgovornost. Indirektno kreiraju misljenje i stavove ljudima sa poluinformacijama, lazima, istinama. Frcaju tekstovi, lazni, tacni, napumpani, navodeci, forsirajuci i stvara se bazen informacija od kojih puca glava. Ljudi su izludjeni.

Objasnio covjek:

Lao Ce wrote: ↑30/11/2020 11:53 Bakire, jesi ovo ti

Jadan čovjek, u potpunosti te razumijem. Nemoj zaboraviti promijeniti pelene

GAU8 wrote: ↑30/11/2020 12:01

Lao Ce wrote: ↑30/11/2020 11:53 Bakire, jesi ovo ti

Jadan čovjek, u potpunosti te razumijem. Nemoj zaboraviti promijeniti pelene

Gdje je nestao onaj filozof, kakve su ovo djetinjarije nivoa osnovaca....

Lao Ce wrote: ↑30/11/2020 12:03

GAU8 wrote: ↑30/11/2020 12:01

Lao Ce wrote: ↑30/11/2020 11:53 Bakire, jesi ovo ti

Jadan čovjek, u potpunosti te razumijem. Nemoj zaboraviti promijeniti pelene

Gdje je nestao onaj filozof, kakve su ovo djetinjarije nivoa osnovaca....

Spuštam se na vaš nivo. To je jedino način komunkacije sa vama E moj Bake....

Lao Ce wrote: ↑30/11/2020 09:54 Dženaza za PCR će se klanjati....

https://cormandrostenreview.com/report/ ... PJHgFc35kg

SUMMARY CATALOGUE OF ERRORS FOUND IN THE PAPER
The Corman-Drosten paper contains the following specific errors:

1. There exists no specified reason to use these extremely high concentrations of primers in this protocol. The described concentrations lead to increased nonspecific bindings and PCR product amplifications, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

2. Six unspecified wobbly positions will introduce an enormous variability in the real world laboratory implementations of this test; the confusing nonspecific description in the Corman-Drosten paper is not suitable as a Standard Operational Protocol making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

3. The test cannot discriminate between the whole virus and viral fragments. Therefore, the test cannot be used as a diagnostic for intact (infectious) viruses, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus and make inferences about the presence of an infection.

4. A difference of 10° C with respect to the annealing temperature Tm for primer pair1 (RdRp_SARSr_F and RdRp_SARSr_R) also makes the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

5. A severe error is the omission of a Ct value at which a sample is considered positive and negative. This Ct value is also not found in follow-up submissions making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

6. The PCR products have not been validated at the molecular level. This fact makes the protocol useless as a specific diagnostic tool to identify the SARS-CoV-2 virus.

7. The PCR test contains neither a unique positive control to evaluate its specificity for SARS-CoV-2 nor a negative control to exclude the presence of other coronaviruses, making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

8. The test design in the Corman-Drosten paper is so vague and flawed that one can go in dozens of different directions; nothing is standardized and there is no SOP. This highly questions the scientific validity of the test and makes it unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

9. Most likely, the Corman-Drosten paper was not peer-reviewed making the test unsuitable as a specific diagnostic tool to identify the SARS-CoV-2 virus.

10. We find severe conflicts of interest for at least four authors, in addition to the fact that two of the authors of the Corman-Drosten paper (Christian Drosten and Chantal Reusken) are members of the editorial board of Eurosurveillance. A conflict of interest was added on July 29 2020 (Olfert Landt is CEO of TIB-Molbiol; Marco Kaiser is senior researcher at GenExpress and serves as scientific advisor for TIB-Molbiol), that was not declared in the original version (and still is missing in the PubMed version); TIB-Molbiol is the company which was “the first” to produce PCR kits (Light Mix) based on the protocol published in the Corman-Drosten manuscript, and according to their own words, they distributed these PCR-test kits before the publication was even submitted [20]; further, Victor Corman & Christian Drosten failed to mention their second affiliation: the commercial test laboratory “Labor Berlin”. Both are responsible for the virus diagnostics there [21] and the company operates in the realm of real time PCR-testing.

In light of our re-examination of the test protocol to identify SARS-CoV-2 described in the Corman-Drosten paper we have identified concerning errors and inherent fallacies which render the SARS-CoV-2 PCR test useless.

CONCLUSION
The decision as to which test protocols are published and made widely available lies squarely in the hands of Eurosurveillance. A decision to recognise the errors apparent in the Corman-Drosten paper has the benefit to greatly minimise human cost and suffering going forward.

Is it not in the best interest of Eurosurveillance to retract this paper? Our conclusion is clear. In the face of all the tremendous PCR-protocol design flaws and errors described here, we conclude: There is not much of a choice left in the framework of scientific integrity and responsibility.